There is a culture of bacteria with 1 x 10 to the 8 cells ml-1.?

Using serial dilutions, how would you dilute this culture to obtain a suspension with 50 cells ml-1?





I need it ASAP please. Thanx!

There is a culture of bacteria with 1 x 10 to the 8 cells ml-1.?
100,000,000:50


10,000,000:5


2,000,000:1 is your dilution factor.





I assume you would want to keep these sterile? So use falcon tubes with 9 mls of Lb in each to do the dilutions:


the more dilutions you do, the more accurate it is..





1 ml into 9 mls - do this 6 times, but in the last dilution transfer 2 mls into 8 mls..





if you feel lazy, you can decrease the numer of dilutions by transfering 100 ul into 10 mls, and doing it only 3 times..





up to you..