Im trying to tag strains of E. coli with luciferase, and figured out a way to get the eukaryotic gene (luc) into the prokaryotic chromosome... but need help with how to stabilize the bacterial chromosome integration of the "L- pr" (L=lambda) promotor into the genome.
I need some sort of delivery vector.
Trying to tag a Gram - Bacteria using biopigmentation, ideas on stabilizing chromosomal integration?
I guess I don't fully understand what you're asking. Can you not just find a vector that would work with your sequence? Design linker primers? I dunno
lady slipper
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