not necessaily strict
How can we culture anaerobes(bacteria) without using CO2 incubator?
If you have a container with a tight sealing lid, you can put a lit candle in with your plates, and that will burn up most of the available oxygen in the container.
It's not a complete anaerobic chamber, for that you would need commercially prepared packets. CO2 incubator are not anaerobic, they are usually just ran at a 5-10% concentration of the gas, as this aids in growth of some bacteria.
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