Saturday, April 24, 2010

A recombinant plasmid gets inside a bacteria by..a,b,c,d?

a.inducing mutations


b.injecting itself into the cell


c.transformation


d.recombing with the cell

A recombinant plasmid gets inside a bacteria by..a,b,c,d?
transformation.
Reply:Transformation.





You know this because the process of transformation involves inserting fragmented DNA sequences into a vector (ie. a plasmid) and then inserting the vector (plasmid) into a host.
Reply:transformation


Does rinsing vegetables with water remove bacteria?

If it is pointless to rinse your hands without soap, does rinsing vegetables with water do anything?

Does rinsing vegetables with water remove bacteria?
It does remove bacteria when u rinse in water, but the extent of removal is dependant on the surface of the vegetable. For example we will take tomatoes, it has wax coat on its surface and even if u wash/rinse for 100times the bacteria sustains on it. The rinsing is required to remove dirt and dirt is one of the sources of bacterial growth.
Reply:Rinsing removes dirt, dust, mold spores, nematode eggs and other nasty microscopic things.
Reply:depends what type of bacteria. If you want to be sure most are removed, then boil 'em veggies. Then again, bacteria you'd find on vegetables is harmless. You're also likely to find bacteria on the fork you use to eat those veggies. Really not much you can do, except for lap testes. Might i suggest performing a streak plate test.
Reply:rinsing does not remove the bacteria its the cooking that does it. Washing gets the dirt and any kinds of pesticides that could still be on the food.
Reply:No, rinsing in water will not remove bacteria. It will only clean off all mud, dirt, dust, pesticides, fertilisers, etc. If the water you use is not pure, then the bacteria from that could get onto the vegetables and make it more 'impure'. Cook vegs well to kill as much bacteria as possible.
Reply:Hi





Yes it does... even rinsing your hands will remove much of the bacteria on them even without soap..





But it will not get it all.. warm water is better than cold also





The best vegetation wash is a store bought one made for that or you can also make your own at home





here is just one recipe








1. Fill your kitchen dish pan with cold water.


2. Add 4 Tbs of salt and juice of 1/2 fresh lemon.


(This makes a diluted form of hydrochloric acid)


3. Soak fruits %26amp; vegetables 5 - 10 minutes.


4. Soak leafy green vegetables for 2 - 3 minutes.


5. Soak berries 1 - 2 minutes.


6. Rinse in cold water after soaking.





Some use vinegar other baking soda over salt..





E coli is the biggest threat and any washing you do helps!





Good Luck


Wismom
Reply:yes, the mechanical action removes bacteria....











my question is, which bacteria are you so afraid of?








what's with all the germ-phobia lately... antibacterial soaps, people friggin scrubbing vegetables, friggin HEPA air filters, antibacterial glass cleaner, etc. etc...





don't people realize you'd be dead without bacteria... they live EVERYWHERE... ALL OVER YOUR SKIN... YOUR MOUTH, IN YOUR INTESTINES... ohhhhhhhhhhh goodnessss what shall we do!!!

yoga

Is Lyme disease a virus or bacteria?

Lyme disease, or borreliosis, is an emerging infectious disease caused by bacteria from the genus Borrelia.[1] The vector of infection is typically the bite of an infected black-legged or deer tick, but other carriers (including other ticks in the genus Ixodes) have been implicated.[2] Borrelia burgdorferi is the predominant cause of Lyme disease in the US and Borrelia afzelii and Borrelia garinii are in Europe.





The disease presentation varies widely, and may include a rash and flu-like symptoms in its initial stage, then musculoskeletal, arthritic, neurologic, psychiatric and cardiac manifestations. In a majority of cases, symptoms can be eliminated with antibiotics, especially if treatment begins early in the course of illness. Late or inadequate treatment often leads to "late stage" Lyme disease that is disabling and difficult to treat. Controversy over diagnosis, testing and treatment has led to two different standards of care

Is Lyme disease a virus or bacteria?
Bacteria
Reply:Lyme disease, is a emerging infectious disease caused by bacteria from the Borrelia.
Reply:A virus that spreads from ticks.
Reply:BACTERIA


Lyme disease, or borreliosis, is an emerging infectious disease caused by bacteria from the genus Borrelia.
Reply:Lyme disease, or borreliosis, is an emerging infectious disease caused by bacteria from the genus Borrelia. The vector of infection is typically the bite of an infected black-legged or deer tick, but other carriers (including other ticks in the genus Ixodes) have been implicated. Borrelia burgdorferi is the predominant cause of Lyme disease in the US and Borrelia afzelii and Borrelia garinii are in Europe.





I hope it helps!
Reply:Lyme disease is caused by the bacterium Borrelia burgdorferi and is transmitted to humans by the bite of infected ticks most likely deer ticks but other ticks can give it too you.





be very careful when in tall grass and wooded areas.





if you feel flu like symtoms and have an odd rash (some people dont get the rash) see a doctor too treat it early otherwise it will be hard to treat and will need iv antibiotics.
Reply:Is this disease caused by germ warfare/contaminated vaccines. Look up research by Prof Donald W Scott, Dr Garth Nicholson and Dr James Martin and judge for yourself.





Regards, Jacqui


Does Anti-bacterial hansoap kill more bacteria than non-antibacterial hansoap?

Anti-bacterial soaps probably "kill" more bacteria than non-antibacterial soap, the important apsect of soap is that it is used to "remove" bacteria from your hands. Therefore the technique and time spent washing you hands is more important.





Soaps (both liquid and bar) rely on the mechanical scrubbing of you hands to remove germs. The soap acts as a detergent that breaks down water tension to "make water wetter" This makes it hard for bacteria to "stick" to you hands.





Just a word about antibiotic liquid soap. The active ingredient in most liquid soap is Triclosan. In the mid- to late-90s, Triclosan was thrown into any and every consumer product to make it "antibacterial" (soaps, plastics, even steering wheels). This misuse and overuse of triclosan has the potential to select for triclosan resistant bacteria. One of my former professors who is now head of National Antimicrobial Resistance Monitoring Systems (NARMS) for the FDA is a staunch opponent of the overuse of Triclosan. When he would question the effectiveness of Triclosan, the reply of the industries using triclosan was always "We do not make any claim of the effectiveness of Triclosan against bacteria". Ok, then why does your product say "Antibacterial"?





I am not trying to make you take a position on Triclosan but you, and the public in general, should be aware of the controversy of the use of it in everyday products

Does Anti-bacterial hansoap kill more bacteria than non-antibacterial hansoap?
A little, perhaps. But it also dries out your skin.
Reply:Yes of course it does
Reply:Hello Mille,


Based on various studies in this topic, the hand washing technique and the time spent on the hand wash matters more than the type of soap/hand wash used.


Although, many hand wash liquids claim that it contains moisturisers many of them dry your hand especially if you wash your hands very frequently. It causes the skin become dry and cracked. It increases the chances of bacterial invasion through the micro-cracks. Hence, it is mandatory to use moisturising hand cream after every hand wash to keep the skin supple.


Regardless of the type of soap/hand wash, spending 30 seconds on hand wash proved to be effective which removes most of the microbes from the hands.


Hope, this helps.
Reply:no

ivy

What is poision secreted by pathogenic bacteria?

this is for biology

What is poision secreted by pathogenic bacteria?
Toxins





Angel
Reply:toxin


One bad use or consequence of bacteria?

This 1 is also 4 biology, so once again I need this ASAP

One bad use or consequence of bacteria?
A bad consequence of bacteria? How about food poisoning, like E. coli and salmonella, makes people sick, and kills some.
Reply:Bacterial Meningitis tends to kill people...is that bad enough?


What are the good things about bacteria?

*name four please*

What are the good things about bacteria?
I love bacteria!!!


They improve the soil so plants do better!


The good bacteria in my intestines keep the bad bacteria at bay to I don't get taco trots!


They get rid of solid waste in my septic system so my yard smells nice!


The good bacteria in my private areas kills yeast so I don't feel bad or stink.
Reply:it helps decompose stuff


it is curable with antibiotics


people die from eating e coli bacteria


and it makes your s hit smell better
Reply:There is a bacteria in yogurt that is good for you! It's a live bateria


Good or even drinkable algae and bacteria?

If so list some facts and explanations

Good or even drinkable algae and bacteria?
yogurt and milk





yogurt is a dairy product produced by bacterial fermentation of milk. Fermentation of the milk sugar (lactose) produces lactic acid, which acts on milk protein to give yogurt its texture and its characteristic tang.





Yogurt has nutritional benefits beyond those of milk: people who are lactose-intolerant can enjoy yogurt without ill effects, because the lactose in the milk precursor is converted to lactic acid by the bacterial culture. The absence of lactose bypasses the affected individuals' need to process the milk sugar themselves.





Yogurt also has medical uses, in particular for a variety of gastrointestinal conditions, and in preventing antibiotic-associated diarrhea. One study suggests that eating yogurt containing L. acidophilus helps prevent vulvovaginal candidiasis, though the evidence is not conclusive.[4]
Reply:just the sound of it makes me sick
Reply:Spirulina, an alga is often consumed in the form of medicine.

fuchsia

How would u research an unknown bacteria?

Lets say its similar to the cholera bacterium. This is just a general question.

How would u research an unknown bacteria?
In addition to colony (size, pattern, color etc.), morphology and structure, gram stain, and biochemical characteristics, I would attempt to find some universal primers to study ribosomal RNA for classification. Whether the primers work or not will lead you to the next step.
Reply:Put it in a search engine...... like yahoo, or google, wikipedia,





DUH!
Reply:if you mean in the internet they go to a search engine, if you mean as a laboratory work I would get all kinds of bacteria I can find and they but in a mic next to the unknown one and make a list of the things they have in common, at least it would help to classify the bacteria...


good luck
Reply:First isolate it, i.e. attempt to grow it in a petri dish. Once they get growing, transfer samples to different nutrition media, such as lacking certain nutrients.


Do gram staining, check them out under a microscope with more staining techniques, subject them to different temperatures. Looking at the spot they were found at can also be helpful.
Reply:Unknown? As in previously undiscovered or one you know nothing about.





Actually it has become very easy to find new bacteria. It has become a fad among microbiologist working on their PhD's. It is fairly easy to find a new bacteria. All you have to do is look in a place or substrate or soil or mud just about anywhere. Find the bacteria, check it out, see if it has been described. There are many millions of bacteria that have not been identified.





People know these critters exist but up until recently nobody has had much interest in finding it. Generally because we know which ones are harmful to humans, the rest, if they have not proved harmful have been ignored.





But, with the onslaught of "Green" research and finding alternatives to fuel, like bio fuel, and alternatives to cleaning up things like oil spills, there is a renewed interest in bacteria.





But researching a "unknown" means you gotta start form the very being. One of the most important steps is determining its DNA. That's how bacteria are cataloged or classified.
Reply:I would inoculate the bacteria on to petri dish and incubate it for few days and see how the colonies turn out...colonies morphology,margin,elevation,color gives an idea as to which bacteria it could be...





then go for Gram staining to confirm


How would u research an unknown bacteria?

Lets say its similar to the cholera bacterium. This is just a general question.

How would u research an unknown bacteria?
In addition to colony (size, pattern, color etc.), morphology and structure, gram stain, and biochemical characteristics, I would attempt to find some universal primers to study ribosomal RNA for classification. Whether the primers work or not will lead you to the next step.
Reply:Put it in a search engine...... like yahoo, or google, wikipedia,





DUH!
Reply:if you mean in the internet they go to a search engine, if you mean as a laboratory work I would get all kinds of bacteria I can find and they but in a mic next to the unknown one and make a list of the things they have in common, at least it would help to classify the bacteria...


good luck
Reply:First isolate it, i.e. attempt to grow it in a petri dish. Once they get growing, transfer samples to different nutrition media, such as lacking certain nutrients.


Do gram staining, check them out under a microscope with more staining techniques, subject them to different temperatures. Looking at the spot they were found at can also be helpful.
Reply:Unknown? As in previously undiscovered or one you know nothing about.





Actually it has become very easy to find new bacteria. It has become a fad among microbiologist working on their PhD's. It is fairly easy to find a new bacteria. All you have to do is look in a place or substrate or soil or mud just about anywhere. Find the bacteria, check it out, see if it has been described. There are many millions of bacteria that have not been identified.





People know these critters exist but up until recently nobody has had much interest in finding it. Generally because we know which ones are harmful to humans, the rest, if they have not proved harmful have been ignored.





But, with the onslaught of "Green" research and finding alternatives to fuel, like bio fuel, and alternatives to cleaning up things like oil spills, there is a renewed interest in bacteria.





But researching a "unknown" means you gotta start form the very being. One of the most important steps is determining its DNA. That's how bacteria are cataloged or classified.
Reply:I would inoculate the bacteria on to petri dish and incubate it for few days and see how the colonies turn out...colonies morphology,margin,elevation,color gives an idea as to which bacteria it could be...





then go for Gram staining to confirm


What is the definition for undersirable bacteria?

It usually refers to pathogenic bacteria (ones that can cause illness) or pathogenic levels of normal flora (bacteria normally harmless unless in significant numbers to cause an infection)

What is the definition for undersirable bacteria?
Undesirable bacteria is responsible for food spoilage. This type of bacteria may not make you sick, but they show themselves by the use of odors, sticky or slimy surfaces and discoloration.


Can anyone give me information on bacteria?

Bacteria, one-celled organisms visible only through a microscope. Bacteria live all around us and within us. The air is filled with bacteria, and they have even entered outer space in spacecraft. Bacteria live in the deepest parts of the ocean and deep within Earth. They are in the soil, in our food, and on plants and animals. Even our bodies are home to many different kinds of bacteria. Our lives are closely intertwined with theirs, and the health of our planet depends very much on their activities.





Bacterial cells are so small that scientists measure them in units called micrometers (µm). One micrometer equals a millionth of a meter (0.0000001 m or about 0.000039 in), and an average bacterium is about one micrometer long. Hundreds of thousands of bacteria would fit on a rounded dot made by a pencil.





Bacteria lack a true nucleus, a feature that distinguishes them from plant and animal cells. In plants and animals the saclike nucleus carries genetic material in the form of deoxyribonucleic acid (DNA). Bacteria also have DNA but it floats within the cell, usually in a loop or coil. A tough but resilient protective shell surrounds the bacterial cell.





Biologists classify all life forms as either prokaryotes or eukaryotes. Prokaryotes are simple, single-celled organisms like bacteria. They lack a defined nucleus of the sort found in plant and animal cells. More complex organisms, including all plants and animals, whose cells have a nucleus, belong to the group called eukaryotes. The word prokaryote comes from Greek words meaning “before nucleus”; eukaryote comes from Greek words for “true nucleus.”

Can anyone give me information on bacteria?
They are small, one cell or multiple cell organisms. They are EVERYWHERE. Mostly they don't cause damage or disease and some are even helpful.

paper bush

Can retroviruses enter and successfully infect bacteria?

Absolutely! Biotechnology has a department devoted the study. Viruses, even retroviruses, can invade a bacteria cell and cause DNA mutation. It's quite fascinating actually. A new type of insulin was created and ultimately produced in vast quantity due to virus infection technology. It''s absolutely possible!!!





I hope this helps!

Can retroviruses enter and successfully infect bacteria?
yes they can.hiv is an example of one.
Reply:yes.. the type of virus that infects a bacteria is called a bacteriophage


Name the scientist who saw lie bacteria in one drop of water &how much ?

anton von leeuwenhock

Name the scientist who saw lie bacteria in one drop of water %26amp;how much ?
Anton Van Leeuwenhoek


Is common cold caused by a bacteria or a virus? if you have been in cold weather?

The common cold is cause by the human rhinovirus and is transmitted from person to person via aerosol droplets or through direct contact with an infected persons bodily fluids. Being out in the cold will not give you a cold, however being out in the cold can weaken your immune system making you more susceptible to the virus.

Is common cold caused by a bacteria or a virus? if you have been in cold weather?
A common cold, meaning the flu?





The flu is a virus.
Reply:A cold is a virus, then you can have complications with a bacterial infection------of the sinus etc
Reply:The common cold is a virus and it is transmitted to you by another person 90% of the time. It is a highly contagious virus.





You need to do something really silly to catch a cold all by yourself. Either getting wet while it is cold or not protecting yourself from the cold.


Also walking in and out from a warm house to the cold outside can do it if you don't cover yourself when you go out.


Coming inside and sitting in front of an air conditionning, still sweating from having been in the heat outside will give you a cold everytime.


My guess would be that the common cold is a chemical reaction in your body when exposed to sudden extreme changes of temperature.





When I go and live in the bush...away from the city people, I never catch a cold. But if a friend with a cold visits me from the city...I will catch it...no worries. That just happened to me 3 weeks ago and I still have not fully recover.
Reply:a virus
Reply:Great question and there is some new informationabout how you get the common cold. The common cold starts out as a virus and often ends us as a bacterial infection.


What is new is a study done in Europe and they took identical groups of men and split them in half. One half of them has their feet soaked in ice cold water for 30 minutes and the other half did not have their feet soaked in ice cold water. The result are interesting. The men who had their feet soaked in ICE COLD WATER had a much higher infection rate of the common cold than the men who did not soak their feet. I don't recall the exact percentages but it was some thing like this: men whose feet were soaked in ICE COLD WATER had a rate of 39% and those who did not have the cold water treatment had a rate of 6%.





This was a small group of around 100 men put it points in the direction that to avoid a cold keep your body warm through out winter and you immune system will be able to protect your better against the common cold.
Reply:it's viral and there is nothing you can take to make it go away it will work its way out of your system in about nine days. till then there are ways of dealing. studies have shown that a cold draft will not give you a cold. it is a virus you got from someone else. if your immune system is strong then your body can fight it or make less potent.
Reply:a virus


Do you know that helicobacter pylori bacteria might have a role in allergy to paprika?

I became allergic to paprika and some spices since two and a half years ago. My blood test proved positive for helicobacter pylori and I got Claritromicine pills for 3 weeks. After the treatment I got rid of allergy to paprika and spices too.

Do you know that helicobacter pylori bacteria might have a role in allergy to paprika?
if you have that bacteria, you need stomach meds....a specific ntibiotic so you wont get an ulcer...or reflux that never ends..

daphne

What is the purpose of soil organism-fungi, worms, and bacteria? How do they help fertilize our soil?

worms poop in the dirt, which is natural fertilizer.


Areas in a salon that are most susceptible to pathogenic bacteria?

Places that are dark warm and damp are breading grounds for all types of bacteria.


HELP! What are the similarites and differences of yeast and bacteria on solid media and under the microscope?

regarding their appearance. Thanks for your help guys.

HELP! What are the similarites and differences of yeast and bacteria on solid media and under the microscope?
it really depends on what bacteria it is





why don't you try googling some images for yeast, and the bacteria you will be viewing


for example here is a picture of what yeast looks like http://biology.clc.uc.edu/fankhauser/Lab...
Reply:I hope you already understand that there are numerous different bacteria and yeast and that there are also numerous different and often very specific growth conditions for each organism. Many different bacteria grow very well in and on the human body but not many different yeast grow very well in those same anatomic areas.





However, if a yeast such as Candida albicans grew next to a bacterial colony such as Staphylococcus epidermidis, the colonies would look rather similar on some media. A beginner could confuse the two types but an experienced microbiologist would quickly notice minor but important differences. Other bacteria would look quite different from both Staphylococcus and that particular yeast. Many yeasts will not grow on the same media as bacteria, so they would be completely overlooked. Still, in general bacterial and yeast colonies could look similar to each other.





They differ significantly microscopically. Bacterial cells are generally much smaller and usually require higher magnification and special staining to acurately describe them. Many yeasts will not stain with typical bacterial stains but they are large enough to observe with lower magnifications and often do not need to be stained in order to observe them. Beyond that, I'd say you need to dig into your microbiology text and study. The answer is much larger than can be described until you have studied much, much more.


How long does it take for pee samples to have bacteria if only refrigerated?

Urine samples should have few bacteria if refrigerated, because bacteria coming off the body grow poorly at low temperature. I would say urine would have fewer bacteria than tap water.





Doesn't it sound like a good science project?

How long does it take for pee samples to have bacteria if only refrigerated?
The amonia should prevent the bacteria from growing.
Reply:It depends on the intensity of refrigeration, but....it should be safe....for over few days.....!! I think
Reply:Urine samples are only good for analysis purposes for less than one hour (for crystal observatioin -- they tend to break down after that). A refrigerated sample to be used for other analyses is probably good for about 12-24 hours.





As long as the sample is in a sterile, enclosed container, there should almost no bacteria in a healthy person/ animal's urine, unless a UTI is suspected. Urine inside the bladder is considered sterile.

camellia